Methods and compositions for enhanced ethanol production
AssigneeDanisco US Inc.
InventorsANDREI MIASNIKOV, VIVEK SHARMA
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Abstract
The instant disclosure is generally related to microbial fermentation, grain processing and ethanol production. Certain embodiments of the disclosure are therefore related to starch-containing materials and the microbial fermentation of such starch-containing materials for the production ethanol. More particularly, certain embodiments are related to novel protease mixtures for use in processing such starch-containing materials in starch to ethanol fermentation processes described herein.
Claims23
A method for producing ethanol from a starch-containing material comprising:
- (a) liquefying a starch-containing material at a temperature above the initial gelatinization temperature of the starch-containing material in the presence of an alpha-amylase,
- (b) saccharifying the liquefied material obtained in step (a) using a saccharifying enzyme, and
- (c) fermenting the material obtained in step (h) with an ethanol production host under suitable conditions for the production of ethanol,
- wherein step (b) and/or step (c) is performed in the presence of an aspartic and serine protease mixture.
A method for producing ethanol from a starch-containing material comprising:
- (a) saccharifying a starch-containing material at a temperature below the initial gelatinization temperature of the starch-containing material using a saccharifying, enzyme, and
- (b) fermenting the material obtained is step (a) with an ethanol production host under suitable conditions for the production of ethanol,
- wherein step (a) anchor step (b) is performed in the presence of an aspartic and serine protease mixture.
A method for producing ethanol from a starch-containing material comprising fermenting a starch-containing material with an ethanol production host under suitable conditions for the production of ethanol, wherein the ethanol production host expresses and secretes a heterologous aspartic protease and a heterologous serine protease, and recovering the ethanol produced.
A method for producing ethanol from a starch-containing material comprising fermenting a starch-containing material with at least two ethanol production hosts under suitable conditions for the production of ethanol, wherein a first ethanol production host expresses and secretes a heterologous aspartic protease and a second ethanol production host expresses and secretes a heterologous serine protease, and recovering the ethanol produced.
A protease composition comprising a mixture of an aspartic protease and a serine protease.
Dependencies are inferred from the claim text.