High-level multiplexing reaction vessel, reagent spotting device and associated methods
AssigneeCepheld
InventorsJennifer Lynn Glass, Michael Quay Chen, John Benjamin Dority, Jonathan Philip Siegrist, Joanna Wong
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Abstract
Reaction vessels, cartridges, devices and methods for facilitating high-level multiplexing are described herein. Such reaction vessels can include a planar frame defining a fluidic path between a first planar substrate and a second planar substrate, a fluidic interface is located at one end of the planar frame with a pair of fluidic ports, a well chamber and a pre-amplification chamber. Devices for spotting reagents in wells of high-level multiplexing reaction vessels and improved reagent solutions are also described herein.
Claims31
A reaction vessel comprising:
- a planar frame defining a fluidic path between a first planar substrate and a second planar substrate;
- and
- a fluidic interface at one end of the planar frame, the fluidic interface comprising a first fluidic port and a second fluidic port, wherein the fluidic path extends between the first and second fluidic ports;
- wherein the fluidic path further includes a well chamber having a plurality of wells, arranged in the planar frame between the first and second substrates, the well chamber disposed along the fluidic path nearer the first fluidic port than the second fluidic port;
- and
- a pre-amplification chamber arranged in the planar frame between the first and second substrate and disposed along the fluidic path nearer the second fluidic port than the first fluidic port.
-101. (canceled)
Dependencies are inferred from the claim text.