Method for enhancing water solubility of target protein by whep domain fusion
AssigneeIndustry-Academic Cooperation Foundation, Yonsei University
InventorsBaik Lin SEONG, Jin Hee LEE, Young Seok KIM, Yu Cheol CHEONG
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Abstract
The present invention relates to a fusion protein for enhancing expression efficiency of a target protein. More specifically, the present invention relates to a glutamyl-prolyl-tRNA synthetase from human (hEPRS) WHEP domain (including WHEP domains TRS-1, TRS-2, and TRS-3 which locate at middle sites of the EPRS protein, and linkers connecting the three domains therethrough). When used as a fusion protein for expressing a target protein in E. coli, the hEPRS WHEP domain according to the present invention enhanced water solubility of the target protein.
Claims13
A peptide for enhancing the expression efficiency of a target protein, comprising the sequence of a domain isolated from human glutamyl-prolyl tRNA synthetase (hEPRS).
Dependencies are inferred from the claim text.