A genetic strain for producing 3-aminoisobutyric acid
AssigneeNanjing Nutrabuilding Bio-Tech Co., Ltd.
InventorsQilin Liao, Jian Zhang
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Abstract
The present invention discloses a S-adenosyl-L-methionine δ24-sterol-C-methyltransferase mutant C24MTgm-M11. Strain MG1655 (ΔptsG ΔfumAC ΔfumB, panD, aspA, C24MTgm) is constructed based on the polynucleotide encoding the enzyme mutant. Strain MG1655 (Δpts GΔfumAC ΔfumB, panD, aspA, C24MTgm-M11) can produce 480 mg/L 3-aminoisobutyric acid under shake flask fermention. Compared to the wild type strain C24MTgm, the strain containing mutant C24MTgm-M11 has a significantly improved ability to produce 5.8 times' 3-aminobutyric acid.
Claims17
A methyltransferase enzyme mutant C24MTgm-M11, which is derived from a wild type enzyme C24MTgm of SEQ ID NO:
- 1, wherein proline 135 is replaced by alanine, leucine 136 is replaced by alanine and phenylalanine 213 is replaced by serine.
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Dependencies are inferred from the claim text.