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US 2023/0028039 A1Published application
Recombinant host cells and methods for the production of d-lactic acid
AssigneeLygos, Inc.
InventorsJeffrey A. Dietrich, Mario Ouellet, Andrew Johnathan Conley
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Abstract
Methods and materials related to producing D-lactic acid are disclosed. Specifically, isolated synthetic or natural nucleic acids, synthetic or natural polypeptides, host cells, and methods and materials for producing D-lactic acid by direct fermentation from carbon sources are disclosed, along with methods of preparing D-lactic acid polymers.
Claims35
A recombinant cell, comprising:
- a heterologous nucleic acid encoding a D-lactate dehydrogenase selected from a sequence having at least 60% amino acid identity with SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, SEQ ID NO:
- 22 or SEQ ID NO:
- 23;
- wherein the heterologous nucleic acid is expressed in sufficient amount to produce D-lactic acid.
12 more elements
A recombinant Pichia kudriavzevii cell, comprising a heterologous nucleic acid encoding D-lactate dehydrogenase selected from SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, SEQ ID NO:
- 22, and SEQ ID NO:
- 23.
7 more elements
Dependencies are inferred from the claim text.