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AssigneesSophion Bioscience A/S, Danmarks Tekniske Universitet
InventorsRie Kjær Christensen, Sandra Wilson, Peder Skafte-Pedersen, Marie Louise Lamb Busk, Niels Bent Larsen, Christoffer Von Halling Laier
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A contractile tissue-based analysis device is provided, in which a strip of contractile tissue is supported by support structure. The support structure comprises a substantially planar base element, and first and second support pillars extending from said base element. An optical detection device is arranged on the side of the base element opposite to said support pillars, and is arranged to capture image data from at least one of the head portions of the support pillars. The motion of the support pillars induced by the strip of contractile tissue can thus be captured from below, i.e. through the planar base element.
A contractile tissue-based analysis device (200), said device (200) comprising at least one support structure (100);
The analysis device (200) according to any one of the preceding claims, wherein said least one fiducial marker (26, 36) also has an extension from the head portion (22, 32) at least in a direction substantially perpendicular to said axis Y-Y.
The analysis device (200) according to any one of the preceding claims, wherein said least one fiducial marker (26, 36) has a primary extension from the head portion (22, 32) which is aligned at an angle of between 30-60°, preferably between 40-50°, more preferably 45° to an axis which extends substantially perpendicular to the plane of the base element (10) along each support pillar (20, 30).
The analysis device according to any one of the preceding claims, wherein said at least one fiducial marker (26, 36) extends from said head portion (22, 32) in a direction away from the base element (10).
The analysis device (200) according to any one of the preceding claims, further comprising a pair of electrodes (201, 202) connected to a power supply and arranged to apply electrical stimulation to the strip of cardiac muscle tissue (210).
The analysis device (200) according to any one of the preceding claims, wherein the support structure (100) of said analysis device (200) comprises a translucent slide (110) arranged on the face of said substantially planar base element (10) opposite to said support pillars (20, 30) and wherein said optical detection device (220) is arranged on the side of the translucent slide (110) opposite to said substantially planar base element (10).
The analysis device (200) according to any one of the preceding claims, wherein said head portions (22, 32) are separated from each other along said axis Y-Y a distance less than or equal to 2 mm.
The analysis device (200) according to any one of the preceding claims, wherein said axis Y-Y is arranged substantially parallel to the plane of said base element (10).
The analysis device (200) according to any one of the preceding claims, wherein at least the planar base element (10) and the translucent slide (110), and preferably the support pillars (20, 30) and the fiducial markers (26, 36), are at least partly transparent to visible light.
The analysis device (200) according to any one of the preceding claims, wherein the head portion (22, 32) has a three-dimensional teardrop shape, with a substantially spherical body which extends to a vertex (25, 35) and wherein the head portions (22, 32) of each support pillar (20, 30) are arranged such that the vertexes (25, 35) of the head portions (22, 32) and the geometric centers of each spherical body, are located along said axis Y-Y, and wherein said vertexes (25, 35) of each head portion (22, 32) are located inward of said geometric centers of each spherical body along said axis Y-Y.
The analysis device (200) according to any one of the preceding claims, further comprising a receptacle (40), said receptacle (40) comprising a basewall (41) and at least one sidewall (42), said basewall (41) comprising at least two openings (41′), wherein each stem portion (21, 31) of the support pillars (20, 30) extends through one of said openings (41′);
The analysis device (200) according to any one of the preceding claims, further comprising at least one dosing means for providing at least one drug to said strip of muscle tissue.
The analysis device (200) according to any one of the preceding claims, wherein said base element (10), said support pillars (20, 30) and said receptacle (40) are formed, preferably 3-D printed, from the same polymer matrix, preferably a compliant polymer matrix, more preferably a hydrogel polymer matrix;
The analysis device (200) according to any one of the preceding claims, wherein said device (200) comprises a plurality of support structures (100) arranged in a planar array (250);
A method for analysing the response of a strip of cardiac muscle tissue (210) to a drug, said method comprising the steps of:
Dependencies are inferred from the claim text.