Fiducials for use in registration of a patterned surface
OwnerJohn S. Vieceli, Hui Han +2inventors
InventorsJohn S. Vieceli, Hui Han, Robert Langlois, Bo Lu
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Abstract
Registration of a patterned flow cell may utilize fiducials comprising sets or groupings of features (e.g., sites, sample wells, nanowells) having known locations and in which the placement of the features is not in accordance with a periodic pattern or is otherwise distinguishable from the periodic pattern of sites present in non-fiducial regions of the flow cell substrate. In certain embodiments the positioning of the sites that are part of the fiducial represent a break or discontinuity in the periodic pattern of sites that are otherwise present on the surface of a patterned flow cell.
Claims25
A patterned flow cell, comprising:
- a substrate;
- a first plurality of sample sites formed on the substrate, wherein the first plurality of sample sites are arranged in a periodic pattern;
- and
- a second plurality of sample sites formed on the substrate, wherein the second plurality of sample sites are arranged in a non-period arrangement, wherein the second plurality of sample sites are fiducials.
A method for registering an image acquired of a patterned flow cell, the method comprising:
- acquiring an image of a patterned flow cell comprising a plurality of sample sites in a periodic pattern and a plurality of fiducials, each fiducial comprising sample sites in a respective non-periodic arrangement;
- comparing image data corresponding to each fiducial to one or more respective templates comprising known location data for the respective non-periodic arrangement of sample sites for each fiducial;
- deriving one or more geometric transforms based on the comparison of the image data to the one or more templates;
- and
- transforming the plurality of sample sites of the patterned flow cell using the one or more geometric transforms.
A patterned flow cell, comprising:
- a substrate;
- a plurality of sample sites formed on the substrate, wherein the plurality of sites are arranged non-periodically over the substrate.
A patterned flow cell, comprising:
- a substrate;
- a first plurality of sample sites formed on the substrate, wherein the first plurality of sample sites are arranged in a first periodic pattern;
- and
- a second plurality of sample sites formed on the substrate, wherein the second plurality of sample sites are arranged in a second periodic pattern different from the first periodic pattern, wherein the difference between the first periodic pattern and the second periodic pattern allow the some or all of the first plurality of sample sites or the second plurality of sample sites to serve as a fiducial.
Dependencies are inferred from the claim text.