Surface-Based Tagmentation
AssigneeIllumina Cambridge Limited
InventorsNiall Anthony Gormley, Avgousta Ioannou, Rosamond Jackson, Natalie Morrell
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Abstract
Presented herein are methods and compositions surface-based tagmentation. In particular embodiments, methods of preparing an immobilized library of fragmented and tagged DNA molecules on a solid surface are presented. In particular embodiments, the solid surface comprises immobilized transposomes in a dried format, suitable for reconstitution upon contact with liquid, such as a liquid sample.
Claims19
A method of transposome-mediated tagmentation of single-stranded target nucleic acids comprising:
- (a) applying a single-stranded nucleic acid to a solid support comprising an immobilized capture sequence under conditions whereby the single-stranded nucleic acid hybridizes to the capture sequence;
- (c) extending the immobilized capture sequence to obtain a double-stranded nucleic acid;
- and
- (d) applying a transposome complex to the solid support under conditions suitable for tagmentation of the double-stranded nucleic acid.
A lateral flow device for tagmentation comprising a solid support comprising:
- i. a sample deposition region;
- ii. a buffer region;
- and
- iii. a tagmentation region comprising immobilized transposome complexes;
- wherein the solid support is configured for sample migration via capillary action from the sample deposition region to the tagmentation region.
Dependencies are inferred from the claim text.