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AssigneeGlaxosmithkline Biologicals SA
InventorsLeigh Anne IHNKEN, Joel KARPIAK, Stefan Jochen KEMMLER, Michael Thomas KOWARIK, Joel MELBY, Anne OLLIS, Julien Laurent QUEBATTE
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The present disclosure provides mutated PglB oligosaccharyltransferase enzymes that have the ability to efficiently catalyze the transfer of a saccharide from a lipid carrier to an asparagine reissue in a glycosylation motif on a protein. Also provided are polynucleotides encoding the mutated PglB oligosaccharyltransferase enzymes, host cells capable of expressing the engineered PglB oligosaccharyltransferase enzymes, and methods of using the engineered PglB oligosaccharyltransferase enzymes to make N-glycosylated proteins. Also provided are N-glycosylated proteins that are made using the engineered PglB oligosaccharyltransferase enzymes.
A PglB oligosaccharyltransferase (OST) polypeptide comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% identical to the amino acid sequence set out in SEQ ID NO:1 or 2 or a functional fragment thereof, wherein the PglB oligosaccharyltransferase polypeptide amino acid sequence includes the feature that:
A PglB from Campylobacter coli (PglBC. coli) wherein the residue corresponding to amino acid X57 of SEQ ID NO:12 is substituted with a different amino acid to that found at that position in SEQ ID NO:12;
Dependencies are inferred from the claim text.