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US 2022/0403337 A1Published application
Cell reprogramming method
AssigneeTakeo SHIMASAKI
InventorsTakeo SHIMASAKI, Satoko YAMAMOTO, Naohisa TOMOSUGI
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Abstract
The present invention pertains to a method for creating reprogramed cells from somatic cells without gene introduction. The method includes a step (a) for culturing somatic cells in a medium containing a histone deacetylase inhibitor, and a step (b) for culturing the cells cultured in step (a) in a medium containing an OCT3/4 transcription stimulating factor to create reprogrammed cells.
Claims15
A method for creating reprogramed cells from somatic cells without gene introduction, the method comprising:
- a step (a) of culturing the somatic cells in a medium containing a histone deacetylase inhibitor;
- and
- a step (b) of culturing cells cultured in the step (a) in a medium containing an OCT3/4 transcription stimulating factor to create the reprogrammed cells.
A kit for reprogramming somatic cells containing the following reagent A and reagent B:
- the reagent A) a reagent for constituting a medium containing 2-mercaptoethanol;
- and
- the reagent B) a reagent for constituting a medium containing LIF.
Dependencies are inferred from the claim text.