Methods for Extracting Proteins from a Blood-Based Material
AssigneePlasma Technologies, LLC
InventorsEugene Zurlo, Dennis Curtin, Klaus Peter Radtke
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Abstract
Methods of producing multiple protein products from blood-based materials including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins are described herein. The inventive methods include steps of fractionation that utilize a combination of salt and organic solvent. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields. The sequence of process steps can be selected to obtain multiple products from various in-process materials, such as supernatants, pastes, chromatography flow-though, and chromatography washes.
Claims28
A method of treating an aqueous solution comprising a plurality of proteins, wherein the plurality of proteins comprises a protein of interest, comprising contacting the plurality of proteins with a salt and an organic solvent, thereby inducing formation of a supernatant and a precipitate from the aqueous solution comprising the plurality of proteins, wherein the protein of interest is included in one of the supernatant and the precipitate.
A method of treating an aqueous solution comprising a plurality of proteins, wherein the plurality of proteins comprises a protein of interest, comprising contacting the plurality of proteins with a precipitant and an organic solvent, thereby inducing formation of a supernatant and a precipitate from the aqueous solution comprising the plurality of proteins, wherein the protein of interest is included in one of the supernatant and the precipitate.
Dependencies are inferred from the claim text.