Back to results
US 2022/0401957 A1Published application
Information storage using enzymatic dna synthesis and digital microfluidics
AssigneemirOculus Inc.
InventorsMais Jehan JEBRAIL, Foteini CHRISTODOULOU
Life of this patent
Estimating the term…
Abstract
Methods and apparatuses for performing Free-Running Synthesis (FRS) and library preparation steps (e.g., nanopore library preparation) on a cartridge using digital microfluidics (DMF) in a tabletop DMF driver/reader apparatus.
Claims20
A method of performing a de novo enzymatic synthesis using digital microfluidics (DMF), the method comprising:
- placing a cartridge having at least one substrate within an air gap of the cartridge, so that a hydrophobic base of the cartridge is in electrical contact with an array of drive electrodes in the DMF apparatus, wherein a plurality of polynucleotide initiator fragments are tethered to the at least one substrate;
- applying power to the drive electrodes to individually move a series of droplets over the substrate by electrowetting in order to repeat the process of:
- (a) passing a droplet comprising an enzymatic mix including a single dNTP type over the at least one substrate comprising the plurality of polynucleotide initiator fragments;
- (b) incubating a droplet on the at least one substrate to add one or of the single dNTP type to and end of the polynucleotide initiator fragments;
- (c) removing the droplet from the at least one substrate;
- wherein steps (a)-(c) are repeated to synthesize a polynucleotide.
3 more elements
A system for performing a de novo enzymatic synthesis using digital microfluidics (DMF), the system comprising:
- a seat for receiving a cartridge;
- a plurality of drive electrodes within the seat configure to electrically couple with a cartridge;
- and
- a controller having one or more processors, the controller further comprising a memory storing computer-program instructions, that, when executed by the one or more processors, perform a computer-implemented method comprising:
- applying power to the drive electrodes to individually move a series of droplets over a substrate by electrowetting within an air gap of a cartridge received in the seat, wherein the air gap holds at least one substrate comprising a plurality of polynucleotide initiator fragments tethered to the at least one substrate, in order to repeat the process of:
- (a) passing a droplet comprising an enzymatic mix including a single dNTP type over the at least one substrate comprising the plurality of polynucleotide initiator fragments;
- (b) incubating a droplet on the at least one substrate to add one or of the single dNTP type to and end of the polynucleotide initiator fragments;
- (c) removing the droplet from the at least one substrate;
- wherein steps (a)-(c) are repeated to synthesize a polynucleotide.
6 more elements
Dependencies are inferred from the claim text.