Rapid cell culture test device including island structures

A cell analysis method using a cell culture test device having an array structure of a plurality of aligned well units, each of which comprises a first inlet portion through which a mixture solution of a gelling agent-containing liquid medium and a biological agent as a first fluid is introduced, a second inlet portion through which a solvent selected from water, cell culture media, dimethylformamide (DMF), and dimethyl sulfoxide (DMSO) as a second fluid is introduced, a first accommodation space in which the first fluid is accommodated, a second accommodation space in which the second fluid is accommodated, an island structure accommodated in the second accommodation space, and a third inlet portion through which an antibiotic is loaded into the island structure, the method comprising (a) loading the antibiotic into the third inlet portion formed above the island structure to fix the antibiotic to the inner and outer surfaces of the island structure, (b) introducing the first fluid into the first inlet portion, allowing the introduced first fluid to flow into the first accommodation space, and gelling the mixture solution to form a solid thin film, (c) introducing the second fluid into the second inlet portion, allowing the second fluid to be accommodated in the second accommodation space and come into contact with the island structure to disperse or dissolve the antibiotic, and allowing the second fluid to come into contact with the solid thin film of the first fluid in a connection portion between the first accommodation space and the second accommodation space to diffuse the antibiotic into the solid thin film, and (d) observing changes of the biological agent on a single cell basis at the interface between the solid thin film of the first fluid and the second fluid and in the solid thin film of the first fluid.