Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases
AssigneeDanisco US Inc.
InventorsSharief Barends, Cristina Bongiorni, Feng Guo, Lori Ann Maggio-Hall, Lawrence Philip Wackett, Gregory M. Whited
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Abstract
The present disclosure is generally related to the fields of biology, molecular biology, genetics, microbial host cells, industrial enzyme production, and the like. More particularly, certain embodiments of the disclosure are related to microbial host cells for the production of heterologous proteins, which microbial host cells are well-suited for growth in submerged cultures for the large-scale production of heterologous cyanuric acid hydrolases and biuret hydrolases.
Claims30
A host cell expressing a heterologous polynucleotide encoding a cyanuric acid hydrolase (CAH) and/or expressing a heterologous polynucleotide encoding a heterologous biuret hydrolase (BH).
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A method for producing a heterologous cyanuric acid hydrolase (CAH) and/or a heterologous biuret hydrolase (BH) in a host cell, the method comprising:
- (a) obtaining a suitable host cell and introducing into the host cell a polynucleotide expression cassette encoding a heterologous CAH and/or introducing into the host cell a polynucleotide expression cassette encoding a heterologous BH,
- (b) fermenting the host cell under suitable conditions for the expression and production of the heterologous CAH and/or the heterologous BH, and
- (c) isolating the heterologous CAH and/or heterologous BH from the host cell or the host cell fermentation broth.
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A method for producing a heterologous cyanuric acid hydrolase (CAH) and/or a heterologous biuret hydrolase (BH) in a host cell, the method comprising:
- (a) obtaining a suitable host cell and introducing into the host cell a polynucleotide expression cassette encoding a heterologous CAH and/or introducing into the host cell a polynucleotide expression cassette encoding a heterologous BH,
- (b) fermenting the host cell under suitable conditions for the expression and production of the heterologous CAH and/or the heterologous BH,
- (c) lysing the host cells after fermenting, and
- (d) at least partially purifying the CAH and/or BH from the lysed host cell fermentation broth.
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Dependencies are inferred from the claim text.