Production of oligosaccharides
AssigneeGinkgo Bioworks, Inc.
InventorsSudeep Agarwala, Michael G. Napolitano
Life of this patent
Estimating the term…
Abstract
The disclosure relates to methods and compositions for the production of fructans using sucrose:sucrose 1-fructosyl-transferase (1-SST), fructan:fructan 1-fructosyltransferase (1-FFT), and/or sucrose fructan-6-fructosyltransferase (6-SFT) enzymes.
Claims40
A host cell that comprises one or more heterologous polynucleotides encoding:
- a) a sucrose:sucrose 1-fructosyltransferase (1-SST) enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 1 or SEQ ID NO:
- 24;
- b) a fructan:fructan 1-fructosyltransferase (1-FFT) enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 7 or SEQ ID NO:
- 31;
- and/or
- c) a sucrose:fructan-6-fructosyltransferase (6-SFT) enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 13 or SEQ ID NO:
- 38.
A method of producing a fructan, comprising contacting sucrose with one or more of:
- a) a sucrose:sucrose 1-fructosyltransferase (1-SST) enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 1 or SEQ ID NO:
- 24;
- b) a fructan:fructan 1-fructosyltransferase (1-FFT) enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 7 or SEQ ID NO:
- 31;
- and
- c) a sucrose:fructan-6-fructosyltransferase (6-SFT) enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 13 or SEQ ID NO:
- 38.
A method of producing a fructan, comprising:
- a) contacting sucrose with a sucrose:sucrose 1-fructosyltransferase (1-SST) enzyme to produce kestose;
- and
- b) contacting the kestose with a fructan:fructan 1-fructosyltransferase (1-FFT) enzyme and/or a sucrose:fructan-6-fructosyltransferase (6-SFT) enzyme to produce the fructan.
A host cell that comprises one or more heterologous polynucleotides encoding:
- a) a sucrose:sucrose 1-fructosyltransferase (1-SST) enzyme comprising an amino acid sequence that is at least 90% identical to a sequence selected from SEQ ID NOs:
- 1-4 and 24-28;
- b) a fructan:fructan 1-fructosyltransferase (1-FFT) enzyme comprising an amino acid sequence that is at least 90% identical to a sequence selected from SEQ ID NOs:
- 7-10 and 31-35;
- and/or
- c) a sucrose:fructan-6-fructosyltransferase (6-SFT) enzyme comprising an amino acid sequence that is at least 90% identical to a sequence selected from SEQ ID NO:
- 13-21 and 38-52.
A method of producing a fructan, comprising contacting sucrose with one or more of:
- a) a sucrose:sucrose 1-fructosyltransferase (1-SST) enzyme comprising an amino acid sequence that is at least 90% identical to a sequence selected from SEQ ID NOs:
- 1-4 and 24-28;
- b) a fructan:fructan 1-fructosyltransferase (1-FFT) enzyme comprising an amino acid sequence that is at least 90% identical to a sequence selected from SEQ ID NOs:
- 7-10 and 31-35;
- and
- c) a sucrose:fructan-6-fructosyltransferase (6-SFT) enzyme comprising an amino acid sequence that is at least 90% identical to a sequence selected from SEQ ID NOs:
- 13-21 and 38-52.
Dependencies are inferred from the claim text.