High-yield peroxide quench-controlled polysaccharide depolymerization and compositions thereof
AssigneeBCD Bioscience, Inc.
InventorsMatthew Joseph AMICUCCI, Cory Glen VIERRA, Riley Anne DREXLER, Kevin Patrick WORRELL, Yiyun LIU
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Abstract
Provided are methods for cleaving polysaccharides, comprising reacting polysaccharides with a Fenton's reagent, and cleaving the treated polysaccharides with a nitrogen-based cleavage reagent, which preferably is also a peroxide-quenching agent. Synthetic oligosaccharide compositions produced by such polysaccharide cleaving methods are further disclosed. Such oligosaccharide compositions are shown to provide utility in various aspects including modulating microbial growth and/or microbial or host metabolism.
Claims235
A method for cleaving polysaccharides, comprising:
- reacting polysaccharides in a reaction mixture with a Fenton's reagent, having a peroxide agent and metal ions, to provide treated polysaccharides;
- and
- cleaving the treated polysaccharides with a nitrogen-based cleavage reagent to generate at least one polysaccharide cleavage product and/or oligosaccharide, characteristic of the polysaccharides.
A method for cleaving polysaccharides, comprising:
- reacting polysaccharides in a reaction mixture with a Fenton's reagent, having a peroxide agent and metal ions, to provide treated polysaccharides;
- and
- cleaving the treated polysaccharides with a polysaccharide-cleavage agent in the presence of a peroxide-quenching agent to generate at least one polysaccharide cleavage product and/or oligosaccharide characteristic of the polysaccharides.
A mixture of oligosaccharides produced by a method comprising:
- a) contacting one or more polysaccharide with a Fenton's reagent, comprising a peroxide agent and metal ions to form a mixture;
- b) allowing the Fenton's reagent to react with the polysaccharide for a specified reaction time;
- and
- c) after passage of the specified reaction time of step b, adding a cleavage agent which may also be a peroxide quenching reagent to the mixture,
- wherein the mixture of oligosaccharides is produced.
A method for cleaving polysaccharides, comprising:
- a) contacting one or more polysaccharide with a Fenton's reagent, comprising a peroxide agent and metal ions to form a mixture;
- b) allowing the Fenton's reagent to react with the polysaccharide for a specified reaction time;
- and
- c) after passage of the specified reaction time of step b, adding a cleavage agent which may also be a peroxide quenching reagent to the mixture.
A synthetic oligosaccharide comprising an α-1,4 glucose backbone wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30.
A synthetic oligosaccharide comprising a β-1,4 xylose backbone wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30.
A synthetic oligosaccharide comprising a β-1,4 glucose backbone wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30.
A synthetic oligosaccharide comprising a combination of β-1,4 and β-1,3 glucose backbone wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30.
A synthetic oligosaccharide comprising a β-1,4 galactose backbone wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30.
A synthetic oligosaccharide comprising a β-1,3 galactose backbone wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30.
A synthetic oligosaccharide comprising a β-1,3 glucose backbone wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30.
A synthetic oligosaccharide comprising a backbone of repeating linear β-1,4 mannose wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30.
A synthetic oligosaccharide comprising a β-1,4 xylose backbone wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30.
A synthetic oligosaccharide comprising a β-1,4 galactose backbone wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30.
The synthetic oligosaccharide of claim 195, wherein the synthetic oligosaccharide comprises β-1,4 linked galactose in linear repeating chain.
A synthetic oligosaccharide comprising a backbone with both β-1,4 mannose and β-1,4 glucose wherein the total number of monomers in the synthetic oligosaccharide ranges from 3 to 30.
A synthetic oligosaccharide generated from corn fiber.
Dependencies are inferred from the claim text.