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AssigneeRegeneron Pharmaceuticals, Inc.
InventorsJikang Wu, Hongxia Wang, Haibo Qiu, Ning Li
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Disclosed are methods for improving compound detection and characterization. Methods for characterizing a sample are disclosed. The methods can include providing a sample to a liquid chromatography system capable of sample separation to generate sample components; analyzing sample components by multiplexed targeted selected ion monitoring (SIM) to generate an inclusion list; and performing iterative mass spectral data-dependent acquisition (DDA) from the inclusion list, to identify individual sample components thereby characterizing the sample. In one example, multiplexed targeted SIMs and iterative MS2 DDA acquisition is used to increase robust compound identification for cell culture medium analysis.
1-36. (canceled)
A method of characterizing a sample, comprising:
The method of claim 1, wherein analyzing the fragmented sample by multiplexed targeted selected ion monitoring (SIM) to generate an inclusion list comprises utilizing an ion trap or orbitrap mass analyzer.
The method of claim 1, wherein performing iterative mass spectral DDA comprises utilizing an ion trap or orbitrap mass analyzer fitted with a segmented quadrupole mass filter.
The method of claim 1, wherein analyzing sample components by multiplexed targeted selected ion monitoring (SIM) to generate an inclusion list comprises segmenting mass to ratio window settings in which multiple segments are included and each segment has multiple windows.
Dependencies are inferred from the claim text.