Assessment of disease risk factors in correlation with neu5gc ingested in food
OwnerVered PADLER-KARAVANI, Salam BASHIR +2inventors
InventorsVered PADLER-KARAVANI, Salam BASHIR, Pilar GALAN, Leopold FEZEU
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Abstract
The present invention discloses methods of identifying subject having an increased risk to develop an N-glycolylneu-raminic acid (Neu5Gc) related disease, methods for assessment risk factors related to a consumption of Neu5Gc from food and methods of predicting the likelihood of developing of Neu5Gc related disease or disorder.
Claims21
1-36. (canceled)
A method for determining an antibody repertoire score in a biological sample obtained from a subject comprising:
- (i) evaluating the repertoire of the anti-Neu5Gc antibodies in the biological sample obtained from the subject;
- and
- (ii) calculating a ratio of antibodies against Neu5Gcα2-3-linked glycans to antibodies against Neu5Gcα2-6-linked glycans,
- wherein the calculated ratio is the antibody repertoire score and wherein the antibody repertoire score value above a control value is indicative to increased likelihood of developing a Neu5Gc related disease or disorder, optionally wherein evaluating the repertoire of the anti-Neu5Gc antibodies in the biological sample comprises determining the amount of antibodies against a set of Neu5Gcα2-3-linked glycans and the amount of antibodies against a set of Neu5Gcα2-6-linked glycans in said biological sample.
A method for identifying a subject at increased risk of developing a Neu5Gc related disease or disorder comprising:
- (i) calculating the antibody repertoire score according to the method of claim 1;
- and
- (ii) comparing the obtained score to a control antibody repertoire score,
- wherein the value of said antibody repertoire score above the value of the control antibody repertoire score is predictive to increased risk of developing the disease or disorder.
A diagnostic kit for evaluating the repertoire of the anti- Neu5Gc antibodies in a biological sample comprising:
- a set of Neu5Gcα2-3-linked glycans;
- a set of Neu5Gcα2-6-linked glycans;
- and
- means for quantifying the amount of antibodies bound to Neu5Gcα2-3-linked glycans and to Neu5Gcα2-6-linked glycans.
A method for predicting an increased likelihood of developing a Neu5Gc related disease or disorder comprising calculating the antibody repertoire score according to the method of claim 1 and further calculating the intake of Neu5Gs based on a collected data on food consumption habits of a subject and combining the results with the antibody repertoire score to calculate an joined likelihood predictive of developing the disease.
A method for identifying a subject at increased risk of a disease or disorder related to consumption of foods containing Neu5Gc, comprising:
- (i) collecting data on actual food consumption of a subject;
- (ii) calculating the intake of Neu5Gc based on collected data of food consumption habits of the subject;
- (iii) measuring the level of total anti-Neu5Gc antibodies in the serum or plasma of the subject;
- and (iv) analyzing a correlation between Neu5Gc consumption from food and the level of total anti-Neu5Gc antibodies, wherein a positive correlation is predictive of increased risk of the disease.
Dependencies are inferred from the claim text.