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US 2022/0356479 A1Published application
Modified polypeptide of meso-diaminopimelate dehydrogenase and method for producing l-threonine using the same
AssigneeCj Cheiljedang Corporation
InventorsMina BAEK, Seung-ju SON, Su Yon KWON, Imsang LEE, Kwang Woo LEE
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Abstract
The present disclosure relates to a modified polypeptide, in which the activity of meso-diaminopimelate is weakened, and a method for producing L-threonine using the same.
Claims11
A modified polypeptide, in which the amino acid corresponding to the 169th amino acid of SEQ ID NO:
- 1 is substituted with leucine, phenylalanine, glutamate, or cysteine, and which has a sequence homology to the amino acid sequence of SEQ ID NO:
- 1 of 80% or higher and less than 100%, and has an activity of meso-diaminopimelate dehydrogenase.
A microorganism of the genus Corynebacterium, which comprises:
- a modified polypeptide, in which the amino acid corresponding to the 169th amino acid of SEQ ID NO:
- 1 is substituted with leucine, phenylalanine, glutamate, or cysteine, and which has a sequence homology to the amino acid sequence of SEQ ID NO:
- 1 of 80% or higher and less than 100%, and has an activity of meso-diaminopimelate dehydrogenase;
- or a polynucleotide comprising the same.
1 more element
A method for preparing L-threonine, comprising a step of culturing in a medium a microorganism of the genus Corynebacterium comprising a modified polypeptide, in which the amino acid corresponding to the 169th amino acid of SEQ ID NO:
- 1 is substituted with leucine, phenylalanine, glutamate, or cysteine, and which has a sequence homology to the amino acid sequence of SEQ ID NO:
- 1 of 80% or higher and less than 100%, and which has an activity of meso-diaminopimelate dehydrogenase.
Dependencies are inferred from the claim text.