Ehrlichia vaccines and immunogenic compositions
AssigneesResearch Development Foundation, Zoetis Services LLC
InventorsJere W. McBRIDE, Paul J. DOMINOWSKI, Suman MAHAN, Jason J. MILLERSHIP, Duncan M. MWANGI, Sharath RAI, Sharon M. WAPPEL
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Abstract
Provided herein are immunogenic compositions that may be used, in some aspects, to induce an immune response against an Ehrlichia such as Ehrlichia cams. In some embodiments, the immunogenic composition comprises an E. canis bacterin and/or adjuvant, such as for example an emulsion or liposomal adjuvant. Related methods such as for diagnosis of or vaccination against ehrlichiosis are also provided.
Claims80
An immunogenic composition comprising:
- (i) at least 1, 2, 3, 4, 5, 6, or all of TRP153, TRP36, TRP140, TRP28, TRP95, TRP 19, or TRP120;
- and at least 1, 2, 3, 4, 5, or more peptides comprising or consisting of a peptide of any one of SEQ ID NOs:1-16;
- (ii) at least 2, 3, 4, 5, or all of TRP153, TRP36, TRP140, TRP28, TRP95, TRP19 or TRP120;
- (iii) an E. canis bacterin and at least 1, 2, 3, 4, 5, 6, or all of TRP153, TRP36, TRP140, TRP28, TRP95, TRP19, or TRP120;
- (iv) an E. canis bacterin and at least 1, 2, 3, 4, 5, or more peptides comprising or consisting of a peptide of any one of SEQ ID NOs:1-16;
- or
- (v) an E. canis bacterin, at least 1, 2, 3, 4, 5, 6, or all of TRP153, TRP36, TRP140, TRP28, TRP95, TRP19, TRP120 and at least 1, 2, 3, 4, 5, or more peptides comprising or consisting of a peptide of any one of SEQ ID NOs:1-16;
- and a pharmaceutically acceptable excipient.
A method of diagnosing exposure to or infection by Ehrlicia or E. canis, comprising:
- a) obtaining a biological sample from a mammalian subject, and
- b) testing the biological sample for immunoreactivity to TRP120, TRP140, and/or TRP36, or a peptide thereof, such as any of SEQ ID NOs:
- 3-11, 14, 15, or 16;
- wherein immunoreactivity to TRP120, TRP140, and/or TRP36 indicates that subject has been exposed to or infected by Ehrlicia or E. canis.
Dependencies are inferred from the claim text.