Controlled pore ceramics chips for high throughput solid state oligonucleotide synthesis
AssigneeNanopec, Inc.
InventorMario Blanco
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Abstract
A nano-structured ceramic film with controlled pore size for the high throughput synthesis of oligonucleotides (DNA and RNA). The film can be cut into chips of predetermined size, and code printed for optical recognition in automated DNA synthesizers. The chips are easily activated under very mild conditions and silanization proceeds uniformly to allow reagents to flow unhindered through its open pores. Mono layer modifications, such as covalently bound silane coupling agents, allows for the addition of universal linkers and improved yields compared to conventional approaches.
Claims14
A substrate for synthesis of an oligonucleotide, comprising:
- a ceramic film having a plurality of pores defining a plurality of interior pore surfaces;
- wherein each of the plurality of pores has a diameter between 50 nanometers and 400 nanometers;
- wherein the plurality of pores are spaced apart from each other a distance between 80 to 650 nanometers;
- and
- wherein the plurality of interior pore surfaces are hydroxylated.
A method of synthesizing an oligonucleotide, comprising the steps of:
- selecting a ceramic film having a plurality of pores defining a plurality of interior pore surfaces that are hydroxylated, wherein each of the pores has an inner diameter that is at least three times a length of the oligonucleotide to be synthesized;
- and
- modifying the interior pore surfaces to attach a universal linker;
- and
- synthesizing the oligonucleotide one nucleotide at time beginning at the universal linker.
Dependencies are inferred from the claim text.