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AssigneeThe Regents of the University of California
InventorsYasuo YOSHIKUNI, Justin B. SIEGEL, Youtian CUI, Wai Shun MAK
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Provided herein, inter alia, are methods, host cells, and vectors for producing 3-hydroxypropionate (3-HP). In some embodiments, the host cells include a recombinant polynucleotide encoding an oxaloacetate decarboxylase (OAADC) and a polynucleotide encoding a 3-hydroxypropionate dehydrogenase (3-HPDH). In some embodiments, the methods include culturing said host cell(s) in a culture medium comprising a substrate under conditions suitable for the recombinant host cell to convert the substrate to 3-HP. Expression of the OAADC and the 3-HPDH results in increased production of 3-HP, as compared to production by a host cell lacking expression of the OAADC and the 3-HPDH.
A method for producing 3-hydroxypropionate (3-HP), the method comprising:
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A method for producing 3-hydroxypropionate (3-HP), the method comprising:
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A recombinant host cell comprising a recombinant polynucleotide encoding an oxaloacetate decarboxylase (OAADC), wherein the OAADC comprises the amino acid sequence of SEO ID NO:1.
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A recombinant fungal host cell comprising a recombinant polynucleotide encoding an oxaloacetate decarboxylase (OAADC), wherein the OAADC comprises the amino acid sequence of SEO ID NO:1.
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A vector comprising:
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Dependencies are inferred from the claim text.