Back to results
US 2022/0348943 A1Published application
Methods and compositions for genome editing via haploid induction
AssigneeMonsanto Technology LLC
InventorsCharles L. Armstrong, Edward J. Cargill, Fenggao Dong, Jonathan C. Lamb, Huachun W. Larue, Richard J. Lawrence, Thomas S. Ream
Life of this patent
Estimating the term…
Abstract
Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.
Claims15
1-114. (canceled)
A method of editing maize genomic DNA, comprising:
- a) introducing into the genome of a maize haploid inducer line a nucleic acid sequence encoding at least one Genome Editing Component (GEC) to produce a first maize plant, wherein the maize haploid inducer comprises an ig1 haploid inducer, and wherein the at least one GEC is introduced into the ig1 haploid inducer;
- b) providing a second maize plant, wherein the second maize plant comprises the maize plant genomic DNA which is to be edited;
- c) crossing the second maize plant with the first maize plant;
- and
- d) selecting at least one haploid progeny produced by the cross of step (c) wherein the haploid progeny comprises the genome of the second maize plant but lacks the genome of the first maize plant, and wherein the genome of the haploid progeny comprises at least one targeted genome modification.
2 more elements
A gene-edited-haploid progeny produced by the method of claim 1, wherein the gene-edited haploid progeny comprises at least one targeted genome modification, and wherein the gene-edited haploid progeny comprises the genome of the second maize plant and lacks the genome of the first maize plant.
Dependencies are inferred from the claim text.