L-rna aptamer cyclization and use thereof
AssigneeCity University
InventorsChun Kit KWOK, Danyang JI
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Abstract
A method of cyclizing an L-RNA aptamer by modifying the aptamer with a 3′ azide and a 5′ alkyne group and using click chemistry reaction-based method. The cyclized L-RNA aptamers have improved binding properties and favour more in vitro/cell applications. Also disclosed is an L-oligonucleotide aptamer having linked ends.
Claims14
A method of linking two terminal ends of oligonucleotide, wherein one of the terminal oligonucleotide ends has a 5′ terminal alkyne;
- and the other one of the terminal oligonucleotide ends has a 3′ azide residue;
- comprising the step of:
- linking the 5′ terminal alkyne to the 3′ azide residue.
An L-oligonucleotide aptamer having linked ends.
A molecule having the structure of 5′-alkyne-L-(rArA) and 3′-azide-L-(rArA) molecule, for appending to the 5′ and 3′end of a linear L-Apt.4-1c,
A method of reducing telomerase activity and inhibiting the rG4-protein interaction by using circular L-RNA aptamer.
Dependencies are inferred from the claim text.