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US 2022/0348912 A1Published application
Compositions and methods for improved gene editing
AssigneeUniversity of Massachusetts
InventorsCraig MELLO, Krishna Sumanth GHANTA
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Abstract
The disclosure provides novel methods and compositions for gene editing. In particular, the disclosure relates to compositions and methods of making nucleic acid donor templates for highly efficient and precise gene editing.
Claims46
A method of enhancing homology directed repair efficiency of an isolated double-stranded nucleic acid donor sequence, the method comprising the steps of:
- 1) denaturing the double-stranded nucleic acid donor sequence to form two single-stranded nucleic acid sequences;
- and
- 2) renaturing the two single-stranded nucleic acid sequences to form the double-stranded nucleic acid donor sequence, thereby enhancing the homology directed repair efficiency of the double-stranded nucleic acid donor sequence,
- wherein the double-stranded nucleic acid donor sequence has enhanced homology directed repair efficiency relative to a double-stranded nucleic acid donor sequence that has not been denatured and renatured.
1 more element
46-95. (canceled)
Dependencies are inferred from the claim text.