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US 2022/0341664 A1Published application
Supercritical drying of chromatographic media
AssigneeMerck Millipore Ltd.
InventorsJohn Boyle, Gary Skarja, Amro Ragheb
Life of this patent
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Abstract
Disclosed are methods for critical point drying a composite material. After exposing the composite material to a supercritical fluid, the composite material dries as the supercritical fluid evaporates with reduced pressure. The composite materials are useful as chromatographic separation media.
Claims17
A method of critical point drying a composite material, comprising the steps of:
- a. providing a composite material comprising:
- i. a support member, comprising a plurality of pores extending through the support member;
- and
- ii. a macroporous cross-linked gel, wherein the macroporous cross-linked gel comprises a polymer formed from a reaction of one or more polymerizable monomers with one or more cross-linkers;
- the macroporous cross-linked gel is located in the pores of the support member;
- and said macropores of the macroporous cross-linked gel are smaller than the pores of the support member;
- b. contacting the composite material with a supercritical fluid at a temperature and a pressure above the critical point of the supercritical fluid, wherein the gaseous and liquid phases are indistinguishable;
- and
- c. reducing the pressure;
- thereby critical point drying the composite material.
7 more elements
A method of critical point drying a composite material, comprising the steps of:
- a. providing a composite material comprising:
- i. a support member, comprising a plurality of pores extending through the support member;
- and
- ii. a macroporous cross-linked gel, wherein the macroporous cross-linked gel comprises a polymer formed from a reaction of one or more polymerizable monomers with one or more cross-linkers;
- the macroporous cross-linked gel is located in the pores of the support member;
- and said macropores of the macroporous cross-linked gel are smaller than the pores of the support member;
- wherein the composite material is wetted with an aqueous solution;
- b. exchanging the aqueous solution with a polar organic solvent, such that the composite material is wetted with the polar organic solvent;
- c. contacting the composite material with a supercritical fluid at a temperature and a pressure above the critical point of the supercritical fluid, wherein the gaseous and liquid phases are indistinguishable;
- and the polar organic solvent is miscible with the supercritical fluid;
- and
- d. reducing the pressure;
- thereby critical point drying the composite material.
10 more elements
A method of critical point drying a composite material, comprising the steps of:
- a. providing a composite material comprising:
- i. a support member, comprising a plurality of pores extending through the support member;
- and
- ii. a macroporous cross-linked gel, wherein the macroporous cross-linked gel comprises a polymer formed from a reaction of one or more polymerizable monomers with one or more cross-linkers;
- the macroporous cross-linked gel comprises a plurality of ligands that are biological molecules or biological ions;
- the macroporous cross-linked gel is located in the pores of the support member;
- and said macropores of the macroporous cross-linked gel are smaller than the pores of the support member;
- wherein the composite material is wetted with an aqueous solution;
- b. exchanging the aqueous solution with a polar organic solvent, such that the composite material is wetted with the polar organic solvent;
- c. contacting the composite material with a supercritical fluid at a temperature and a pressure above the critical point of the supercritical fluid, wherein the gaseous and liquid phases are indistinguishable;
- and the polar organic solvent is miscible with the supercritical fluid;
- and
- d. reducing the pressure;
- thereby critical point drying the composite material.
11 more elements
Dependencies are inferred from the claim text.