Crosslinked Hydrogel for Muscle Stem Cell Culture and Preparation Method and Use Thereof

A preparation method of a crosslinked hydrogel for muscle stem cell culture, comprising:

• dissolving collagen to prepare a solution and adding alginate and heparan sulfate proteoglycan for being uniformly mixed with the collagen solution;
• and adding ε-PL and TGase into the solution, uniformly stirring, and putting a slurry into a mold for crosslinking to obtain the hydrogel;
• and specifically comprising the following steps:
• (1) preparing the collagen solution:
• uniformly mixing and dissolving the collagen with an acetic acid aqueous solution to obtain the collagen solution;
• (2) preparing a collagen/alginate solution:
• adding the alginate to the collagen solution prepared in step (1) and stirring until the alginate dissolves to obtain the collagen/alginate solution;
• (3) preparing a collagen/alginate/heparan sulfate proteoglycan solution:
• adding the heparan sulfate proteoglycan to the collagen/alginate solution prepared in step (2) and stirring until the heparan sulfate proteoglycan dissolves to obtain the collagen/alginate/heparan sulfate proteoglycan solution;
• (4) preparing a first physically crosslinked slurry:
• adding the ε-PL to the collagen/alginate/heparan sulfate proteoglycan solution prepared in step (3) and uniformly stirring to obtain the first physically crosslinked slurry;
• (5) preparing a second enzymatically crosslinked slurry:
• adding the TGase to the first slurry prepared in step (4) and uniformly stirring to obtain the second enzymatically crosslinked slurry;
• and
• (6) preparing a hydrogel:
• putting the slurry obtained in step (5) into a mold, crosslinking for 12-36 h, and demolding to obtain the hydrogel for growth of muscle stem cells.