Burstable liquid storage package for biological materials and valve substitution

A method of fabricating a sealed storage apparatus (100) capable of compactly containing a liquid reagent while acting as a phase-change microvalve, the method comprising:

• a. placing a first material over a mold (210) having a mold cavity;
• wherein the first material is liquid-impermeable, air-impermeable, and resistant to force-induced tearing;
• wherein the first material is penetrated through application of a specialized device;
• b. heating the first material;
• c. pressing, by a stamp (220), the first material such that the first material is pressed into the mold cavity to create a pouch body (110) having an opening, and a pouch cavity (120) disposed within the opening of the pouch body (110);
• wherein a shape of the pouch body (110) is the same as a shape of the mold cavity;
• wherein a shape of the pouch cavity (120) is the same as a shape of the stamp (220);
• d. filling the cavity with the liquid reagent;
• e. placing a sealing adhesive (130) over the opening of the pouch body (110), such that the sealing adhesive (130) seals the liquid reagent within the pouch body (110);
• wherein a material of the sealing adhesive (130) is liquid-impermeable, air-impermeable, and resistant to force-induced tearing;
• wherein the sealing adhesive (130) is penetrated through application of the specialized device;
• and
• f. integrating the sealed storage apparatus (100) into a microfluidic platform (300) such that penetrating the sealed storage apparatus (100) causes the liquid reagent to dispense into the microfluidic platform (300).

A microfluidic centrifugal disk (CD) platform (300) comprising:

• a. one or more reagent chambers (310);
• b. one or more collection chambers (320) fluidly connected to the one or more reagent chambers (310) by one or more microfluidic channels (330);
• and
• c. one or more sealed storage apparatuses (100) disposed within the one or more reagent chambers (310), each storage apparatus (100) comprising a sealed pouch cavity (120) that contains a fluid, wherein each storage apparatus (100) is comprised of a material that is liquid-impermeable, air-impermeable, and resistant to force-induced tearing, wherein the material is penetrated through application of a specialized device, thereby releasing the fluid.