Application of tpk as a target in alzheimer's disease
AssigneeShanghai Raising Pharmaceutical Co., Ltd.
InventorsChunjiu Zhong, Shaoming Sang, Jingjing Wang, Huan Zhang
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Abstract
Provided is use of thiamine pyrophosphokinase TPK as a target in the treatment of Alzheimer's disease; and AD symptoms due to the inhibited TPK can be prevented by promoting the kinase activity and/or expression level of TPK protein in brain with TPK as a target.
Claims22
1-8. (canceled)
A method for constructing an animal model of Alzheimer' s disease, the method comprising introducing a TPK gene knockout into a target animal.
16-33. (canceled)
A targeting vector for constructing an animal model for Alzheimer's disease, characterized in that the vector comprises a Bacterial Artificial Chromosome as a vector, direct loxP sequences inserted at both ends of a sequence homologous to an exon of TPK gene, and a neomycin resistance gene sequence.
A recombinant adeno-associated virus (rAAV) or recombinant Lentivirus comprising a polynucleotide comprising an expression cassette comprising a polynucleotide encoding thiamine pyrophosphokinase (TPK) polypeptide operably linked to a promoter.
A method for preventing or treating Alzheimer's disease, the method comprising administering to a subject a medicament that promotes an expression level of TPK protein and/or kinase activity in the subject's brain.
A method of screening a medicament for preventing and/or treating Alzheimer's disease, the method comprising administering the medicament to a subject and measuring kinase activity and/or expression level of TPK protein in the subject's brain.
Dependencies are inferred from the claim text.