Biosynthesis of alpha-ionone and beta-ionone
AssigneeConagen Inc.
InventorsYisheng Wu, Wanli Lu, Jacob Thomas Courtney, David Nunn, Oliver Yu
Life of this patent
Estimating the term…
Abstract
Provided herein are recombinant nucleic acid molecules, nucleic acid constructs, fusion enzymes, transformed host cells, and methods for making aroma compounds alpha-ionone or beta-ionone.
Claims33
A recombinant microbial production host cell for producing an ionone compound, said host cell comprising at least one nucleic acid construct comprising a first coding sequence, wherein said first coding sequence encodes a fusion enzyme comprising a first domain capable of functioning as a lipid body compartmentalization signal tag fused to a second domain having carotenoid cleavage activity.
(canceled)
-12. (canceled)
-17. (canceled)
-20. (canceled)
A recombinant microbial production host cell for producing a carotenoid compound, said host cell comprising at least one nucleic acid construct comprising a coding sequence encoding a fusion enzyme comprising an oleosin polypeptide fused to a beta-carotene ketolase or a beta-carotene hydroxylase.
-24. (canceled)
-27. (canceled)
(canceled)
A synthetic or recombinant nucleic acid molecule comprising a first nucleic acid sequence and a second nucleic acid sequence, wherein the first nucleic acid sequence and the second nucleic acid sequence together encode a fusion enzyme, said fusion enzyme comprising a first domain capable of functioning as a lipid body compartmentalization signal tag and a second domain having carotenoid 9,10(9′,10′)-cleavage activity.
-38. (canceled)
-52. (canceled)
A fusion enzyme comprising a lipid body compartmentalization signal tag (LBT) domain coupled to a carotenoid cleavage dioxygenase (CCD) domain.
-64. (canceled)
-67. (canceled)
-70. (canceled)
Dependencies are inferred from the claim text.