Biosynthesis of eriodictyol
AssigneeConagen Inc.
InventorsRui Zhou, Junying Ma, Oliver Yu
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Abstract
The present invention relates to methods and transformed host cells for the production of eriodictyol from naringenin via bioconversion.
Claims21
A method of producing a 3′-hydroxylated flavonoid, the method comprising incubating a transformed host cell in a suitable medium comprising a flavonoid for a sufficient time to convert said flavonoid to a 3′-hydroxylated flavonoid, wherein the transformed host cell comprises a synthetic or recombinant nucleic acid molecule comprising a first polynucleotide sequence that encodes a flavonoid 3′-hydroxylase, said flavonoid 3′-hydroxylase comprising:
- i) an amino acid sequence having at least 85% sequence identity to the amino acid sequence of SEQ ID NO:
- 12, ii) the amino acid sequence of SEQ ID NO:
- 20, or iii) an amino acid sequence having at least 80% sequence identity to the amino acid sequence of SEQ ID NO:
- 12 and the amino acid sequence of SEQ ID NO:
- 20.
An isolated recombinant host cell transformed with a nucleic acid construct comprising a first polynucleotide sequence encoding a flavonoid 3′-hydroxylase comprising:
- i) an amino acid sequence having at least 85% sequence identity to the amino acid sequence of SEQ ID NO:
- 12, ii) the amino acid sequence of SEQ ID NO:
- 20, or iii) an amino acid sequence having at least 80% sequence identity to the amino acid sequence of SEQ ID NO:
- 12 and the amino acid sequence of SEQ ID NO:
- 20.
Dependencies are inferred from the claim text.