US 2022/0317137 A1 · Test Kit for Testing Fat-soluble Vitamins in Serum Using High Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

Abstract

The present invention describes a test kit for the detection of fat-soluble vitamins in serum using a method based on high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). The internal standard (IS) solution included in the kit is based on methanol, acetonitrile and isopropyl alcohol solvents. Ammonium acetate is added to the IS solution to make it more stable and last for longer storage time. The application of this kit can significantly improve the recovery and detection sensitivity of vitamins A, E, K1 and K2 in serum without additional sample enrichment, make sample preparation process simpler and more efficient, keep processed samples stable for longer time, and lower the overall cost for more accurate and repeatable test results.

Claims20

Dependent claims

A test kit based on HPLC-MS/MS for detecting fat-soluble vitamin in serum comprising:

an internal standard solution, wherein the internal standard solution comprises stable isotope labeled fat-soluble vitamin and an organic solvent for denaturing protein in the serum, and wherein the solvent includes methanol, acetonitrile and isopropanol.

Dependencies are inferred from the claim text.

Facts

Application

17/238,652Filed 23 Apr 2021

Priority

CN 20211024114271 Apr 2021

Published

6 Oct 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification13 codes

G01N 33/6848
General methods of protein analysis not limited to specific proteins or families of proteins: methods of protein analysis involving mass spectrometry
G01N 33/82
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing:…
G01N 1/4044
Concentrating samples: by chemical techniques; Digestion; Chemical decomposition
G01N 30/88
Column chromatography: integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86

Test Kit for Testing Fat-soluble Vitamins in Serum Using High Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

Life of this patent

Test Kit for Testing Fat-soluble Vitamins in Serum Using High Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

Life of this patent

Abstract

Claims20