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AssigneeChangsha Lvye Biotechnology Co., Ltd.
InventorsWei DONG, Xiaohong XIE, Lixin WEN, Chaoliang WU, Xin LI, Yuanyuan ZHU, Yongyong ZHANG, Jiahao HUANG
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A recombinant vector containing the immunogenic protein of African swine fever virus, a recombinant bacterium and use thereof, and relates to the technical field of gene recombination. The recombinant vector can be used to construct a recombinant Lactobacillus expressing the immunogenic protein of African swine fever virus, and after mixing the Lactobacillus solution that can secrete protein p72 and protein p54, respectively, an oral live bacterial preparation for preventing African swine fever can be prepared. The oral live bacteria preparation prepared by the disclosure can safely, effectively and quickly prevent the infection of African swine fever virus to pigs, and does not contain an immune process.
A recombinant vector containing immunogenic protein of African swine fever virus, wherein the recombinant vector takes Lactobacillus expression vector pVE5523 as basic vector, and the nucleotide sequence encoding the immunogenic protein of African swine fever virus is cloned between EcoRV and SalI restriction sites of the basic vector.
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