Recombinant herpesvirales vector
AssigneeSolid Biosciences Inc.
InventorsPeter Pechan, Alexey Vladimirovich Seregin
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Abstract
The invention described herein provides a recombinant replication-defective vims derived from Herpesvirales order, wherein the virus is characterized by a complete deletion of a gene encoding ICP27, or a functional equivalent gene thereof. The invention also provides production cell lines for such recombinant replication-defective vims, wherein the cell lines have a coding sequence for ICP27 or a functional equivalent thereof, and wherein the coding sequence has no or minimal sequence overlap with the virus characterized by the complete deletion of the gene encoding ICP27. Method of using such recombinant replication-defective vims and production cell lines are also provided.
Claims50
A recombinant replication-defective virus derived from the Herpesvirales order, wherein said virus is characterized by a deletion in a gene encoding ICP27, or a functional equivalent gene thereof, wherein said deletion is at least 1,200 bps in length and leaves no more than 300 bp, 250 bp, 200 bp, 150 bp, 100 bp, 50 bp, 30 bp, 20 bp, 10 bp, 9 bp, 8 bp, 7 bp, 6 bp, 5 bp, 4 bp, 3 bp, 2 bp, 1 bp or 0 bp of the most 3′-end of said gene encoding ICP27 (SEQ ID NO:
- 11), or the functional equivalent gene thereof.
A method of making a recombinant replication-defective virus derived from the Herpesvirales order, wherein said virus is characterized by a deletion in a gene encoding ICP27, or a functional equivalent gene thereof, wherein said deletion is at least 1,200 bps in length and leaves no more than 300 bp, 250 bp, 200 bp, 150 bp, 100 bp, 50 bp, 30 bp, 20 bp, 10 bp, 9 bp, 8 bp, 7 bp, 6 bp, 5 bp, 4 bp, 3 bp, 2 bp, 1 bp or 0 bp of the most 3′-end of said gene encoding ICP27 (e.g., SEQ ID NO:
- 11), or the functional equivalent gene thereof, said method comprising creating said deletion of said gene encoding ICP27 or said functional equivalent gene thereof by homologous recombination in a host cell.
Dependencies are inferred from the claim text.