Compositions and methods for modifying a plant characteristic without modifying the plant genome
AssigneesThe United States of America as represented by the Secretary of Agriculture, Agrosource, Inc.
InventorsRobert G. Shatters, Eddie W. Stover, Randall P. Niedz, Michelle L. Heck, Marco Pitino, Magali Ferrari Grando, Joseph Krystel
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Abstract
The invention relates to methods and compositions for modifying a characteristic of a plant without modifying the plant's genome using one or more cells comprising one or more phytohormone genes and at least one polynucleotide of interest, which one or more phytohormone genes and the at least one polynucleotide of interest are expressed in the one or more cells.
Claims120
A symbiont forming inoculum comprising a polynucleotide encoding a phytohormone biosynthetic enzyme and a polynucleotide of interest, wherein the phytohormone biosynthetic enzyme is at least one cytokinin biosynthetic enzyme and/or an auxin biosynthetic enzyme.
A symbiont comprising a plant cell comprising and expressing a polynucleotide encoding a phytohormone biosynthetic enzyme and a polynucleotide of interest, wherein the phytohormone biosynthetic enzyme is at least one cytokinin biosynthetic enzyme and/or at least one auxin biosynthetic enzyme and the plant cell of the symbiont autonomously divides.
The symbiont of any one of claims 26-42, wherein the plant cell is from a macroalgae, an angiosperm, a gymnosperm or a pteridophyte.
A method of producing a symbiont forming inoculum, the method comprising:
- introducing into a cell a polynucleotide encoding a phytohormone biosynthetic enzyme and a polynucleotide of interest or introducing a polynucleotide encoding a phytohormone biosynthetic enzyme into a transgenic cell that comprises a polynucleotide of interest, wherein the phytohormone biosynthetic enzyme is at least one cytokinin biosynthetic enzyme and/or an auxin biosynthetic enzyme, thereby producing the symbiont forming inoculum, optionally, wherein the cell is a plant cell or a bacterial cell.
A method of producing a symbiont forming inoculum, the method comprising
- (a) (i) introducing into/onto at least one site on a plant (or a part thereof (e.g., explant)) a polynucleotide encoding a phytohormone biosynthetic enzyme and a polynucleotide sequence of interest or transplanting a plant cell comprising the same or inoculating a bacterial cell comprising the same onto at least one site on the plant (or a part thereof), or
- (ii) introducing a polynucleotide encoding a phytohormone biosynthetic enzyme, or transplanting a plant cell comprising the same, or inoculating a bacterial cell comprising the same onto at least one site on the plant (or a part thereof) into/onto at least one site on a plant (or a part thereof), the plant (or a part thereof) of (ii) comprising a polynucleotide sequence of interest,
- wherein the phytohormone biosynthetic enzyme is at least one cytokinin biosynthetic enzyme and/or an auxin biosynthetic enzyme, thereby producing a symbiont on the plant (or part thereof) that comprises the polynucleotide encoding a phytohormone biosynthetic enzyme and the polynucleotide sequence of interest;
- and
- (b) selecting one or more cells from the symbiont on the plant, to provide one or more cells comprising the polynucleotide encoding a phytohormone biosynthetic enzyme and the polynucleotide sequence of interest, thereby producing the symbiont forming inoculum.
The method of 106, wherein the bioactive molecule is a bacteriocin or an antimicrobial peptide and the plant pathogen is a bacterium.
Dependencies are inferred from the claim text.