Rapid diagnostic test component
AssigneeDetect, Inc.
InventorsJonathan M. Rothberg, Isaac Bean, Todd Roswech
Life of this patent
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Abstract
Provided herein, in some embodiments, are rapid diagnostic tests to detect one or more target nucleic acid sequences (e.g., a nucleic acid sequence of one or more pathogens). In some embodiments, the pathogens are viral, bacterial, fungal, parasitic, or protozoan pathogens, such as SARS-CoV-2 or an influenza virus. Further embodiments provide methods of detecting genetic abnormalities. Diagnostic tests comprising a sample-collecting component, one or more reagents (e.g., lysis reagents, nucleic acid amplification reagents), and a detection component (e.g., a component comprising a lateral flow assay strip) are provided.
Claims24
A component of a diagnostic test comprising:
- an absorbent pad at least partially saturated with a first solution, wherein the absorbent pad is fluidly coupled between a reservoir that receives a sample and a readout element, wherein the sample flows between the reservoir and the readout element through the absorbent pad.
A diagnostic test kit comprising:
- a lateral flow assay strip;
- an absorbent pad fluidly connected to the lateral flow assay strip, wherein the absorbent pad is at least partially saturated with a first solution;
- a fluidic channel fluidly connected to the absorbent pad;
- and
- a receptacle fluidly connected to the fluidic channel and configured to receive and fluidly connect to a first reservoir containing a sample, wherein fluidly connecting the first reservoir to the receptacle allows the sample to flow from the first reservoir to the absorbent pad via the fluidic channel.
A method of manufacturing a diagnostic test, comprising:
- placing a readout element and an absorbent pad in a housing such that the readout element is in fluid communication with the absorbent pad;
- at least partially filling the absorbent pad with a first solution;
- and
- providing a vial for receiving a sample from a patient such that the vial may fluidly connect to the housing, thereby enabling the sample to flow to the absorbent pad.
Dependencies are inferred from the claim text.