Improved oligosaccharide production in yeast
AssigneeAmyris, Inc.
InventorsJessica WALTER, Dominic PINEL
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Abstract
Provided herein are genetically modified yeast cells capable of producing one or more human milk oligosaccharides (HMOs) and methods of making such cells. The yeast cells are engineered to comprise a heterologous nucleic acid encoding a transporter protein and one or more heterologous nucleic acids that encode enzymes of a HMO biosynthetic pathway. Also provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering HMOs generated by the yeast cells.
Claims99
A yeast cell genetically modified to produce one or more human milk oligosaccharides, wherein the yeast cell comprises (i) a heterologous nucleic acid encoding a human milk oligosaccharide (HMO) ABC transporter polypeptide;
- and (ii) one or more heterologous nucleic acids that each independently encode at least one enzyme of a human milk oligosaccharide biosynthetic pathway.
A method of genetically modifying a yeast cell to produce one or more human milk oligosaccharides, the method comprising:
- (a) (i) introducing a heterologous nucleic acid encoding an ABC transporter polypeptide into the yeast cell;
- and (ii) introducing one or more heterologous nucleic acids that each independently encode at least one enzyme of a human milk oligosaccharide biosynthetic pathway into the yeast cell;
- or
- (b) introducing a heterologous nucleic acid encoding an ABC transporter polypeptide into the yeast cell, wherein the yeast cell comprises one or more heterologous nucleic acids that each independently encode at least one enzyme of a human milk oligosaccharide biosynthetic pathway into the yeast cell.
The method of any one of claims Error! Reference source not found.79, wherein the one or more human milk oligosaccharides comprise lacto-N-neotetraose.
Dependencies are inferred from the claim text.