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AssigneeMonsanto Technology LLC
InventorsCharles R. DIETRICH, Natalia IVLEVA, Thomas L. SLEWINSKI
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The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits and yield relative to a control plant, such as increased ear area, increased ear volume, increased single kernel weight, increased ear fresh weight, increased ear diameter, increased ear length, increased yield, increased kernels per ear, decreased ear tip void, and decreased ear void. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more CONSTANS (CO) or CONSTANS-like (COL) polypeptides and a transgene encoding one or more MADS-box polypeptides, and can also have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.
A modified corn plant or a plant part thereof comprising 1) a first recombinant expression cassette comprising a transcribable DNA sequence encoding a non-coding RNA for suppression of one or more gibberellic acid 20 (GA20) oxidase genes and/or one or more gibberellic acid 3 (GA3) oxidase genes, 2) a second recombinant expression cassette comprising a first DNA sequence encoding a CONSTANS (CO) or CONSTANS-like (COL) polypeptide, and 3) a third recombinant expression cassette comprising a second DNA sequence encoding a MADS-box polypeptide.
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A method for producing a modified corn plant, the method comprising:
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A modified corn plant comprising 1) one or more mutations or edits at or near one or more endogenous GA20 oxidase and/or GA3 oxidase genes, wherein the expression or activity of the one or more endogenous GA20 oxidase and/or GA3 oxidase genes is reduced relative to a wildtype control plant, 2) a first recombinant expression cassette comprising a coding sequence for a CO or COL polypeptide operably linked to a first plant-expressible promoter, and 3) a second recombinant expression cassette comprising a coding sequence for a MADS-box polypeptide operably linked to a second plant-expressible promoter.
A recombinant DNA construct comprising:
A modified corn plant or a plant part thereof comprising 1) a first recombinant expression cassette comprising a first DNA sequence encoding a CONSTANS (CO) or CONSTANS-like (COL) polypeptide, and 2) a second recombinant expression cassette comprising a second DNA sequence encoding a MADS-box polypeptide.
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Dependencies are inferred from the claim text.