Pod shatter tolerance in brassica plants
AssigneePioneer Hi-Bred International, Inc.
InventorsSarah Atwood, Norbert Brugiere, Igor FALAK, Kevin A Fengler, Siva S. Ammiraju Jetty, Jonathan Myrvold
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Abstract
This disclosure provides methods and compositions for identifying Brassica plants that have a native deletion of the INDEHISCENT gene (BnIND-A) located on chromosome A of B. napus. Also provided are methods of improving one or more agronomic characteristics such as pod shatter and breeding methods for introducing a pod shatter tolerant phenotype in Brassica plants and/or their progeny.
Claims17
A method of identifying a Brassica plant, cell, or germplasm thereof comprising an BnIND-A genomic deletion that contributes to a pod shatter tolerance phenotype, the method comprising:
- a. obtaining a nucleic acid sample from a Brassica plant cell, or germplasm;
- and
- b. screening the sample for genomic sequence comprising a deletion of the BnIND-A gene on chromosome N03, wherein the deleted genomic segment is from about 200 kb to about 310 kb in length and has a deletion start breakpoint corresponding to about position 13,300,000 to 14,915,000 of N03 wildtype reference genome and the deletion end breakpoint corresponds to about position 13,500,000 to 15,250,000 of a N03 wildtype reference genome, and wherein the BnIND-A deletion contributes to pod shatter tolerance phenotype in Brassica.
Dependencies are inferred from the claim text.