Novel compositions and uses thereof
AssigneeColzyx AB
InventorsMatthias MÖRGELIN, Suado Mohamed ABDILLAHI
Life of this patent
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Abstract
The present invention provides a composition comprising: (a) collagen VI or a polypeptide comprising or consisting of an amino acid sequence derived from collagen type VI or a fragment, variant, fusion or derivative thereof; and (b) polylysine. Also provided are pharmaceutical compositions and kits comprising the composition of the invention. Related aspects provide medical devices, implants, wound care products and materials for use in the same associated with the composition of the invention, and methods of their preparation. Also provided are methods and uses of the composition in the treatment and/or prevention of microbial infections and in wound care, and a method of killing microorganisms in vitro.
Claims69
A composition comprising:
- (a) collagen type VI, or a polypeptide comprising or consisting of an amino acid sequence derived from collagen type VI, or a fragment, variant, fusion or derivative thereof, or a fusion of said fragment, variant of derivative thereof;
- and
- (b) polylysine.
A composition according to any one of the preceding claims wherein the collagen type VI or polypeptide is capable of binding to the membrane of the microorganism.
A composition according to any one of the preceding claims wherein the collagen type VI or polypeptide is capable of causing membrane disruption of the microorganisms.
A composition according to any one of the preceding claims which is capable of promoting wound closure.
A composition according to any one of the preceding claims, wherein the collagen type VI or polypeptide is capable of exhibiting an antimicrobial effect greater than or equal to that of LL-37.
A composition according to any one of the preceding claims wherein the microorganisms are Gram-positive or Gram-negative bacteria.
A composition according to any one of the preceding claims wherein the microorganisms are bacteria which are resistant to one or more conventional antibiotic agents.
A composition according to any one of the preceding claims wherein the collagen type VI or polypeptide is substantially non-toxic to mammalian cells.
A composition according to any one of the preceding claims wherein the collagen type VI or polypeptide is capable of exerting an anti-endotoxic effect.
A composition according to any one of the preceding claims wherein the polypeptide is derived from a von Willebrand Factor type A domain.
A composition according to any one of the preceding claims wherein the polypeptide is or is derived from the α1, α2 and/or α3 chain of collagen type VI.
A composition according to any one of the preceding claims wherein the polypeptide is or is derived from the N2, N3 or C1 domain of the α3 chain of collagen type VI.
A composition according to any one of the preceding claims wherein the collagen type VI or polypeptide has a net positive charge.
A composition according to any one of the preceding claims wherein the collagen type VI or polypeptide has at least 30% hydrophobic residues.
A composition according to any one of the preceding claims comprising or consisting of the amino acid sequence selected from the group consisting of SEQ ID NOs:
- 1 to 23, and fragments, variants, fusions or derivatives thereof, and fusions of said fragments, variants and derivatives thereof, which retain an antimicrobial activity of any one of SEQ ID NOs:1 to 23.
A composition according to any one of the preceding claims wherein the polypeptide comprises or consists of a variant of the amino acid sequence selected from the group consisting of SEQ ID NOs:
- 1 to 23.
A composition according to any one of the preceding claims wherein the polypeptide is between 10 and 200 amino acids in length, for example between 10 and 150, 15 and 100, 15 and 50, 20 and 40 or 25 and 35 amino acids in length.
A composition according to any one of the preceding claims wherein the collagen type VI or polypeptide, or fragment, variant, fusion or derivative thereof, comprises one or more amino acids that are modified or derivatised.
A composition according to any one of the preceding claims wherein the collagen type VI or polypeptide is a recombinant polypeptide.
A medical device, implant, wound care product, or material for use in the same, which is coated, impregnated, admixed or otherwise associated with a composition or pharmaceutical composition according to any one of the preceding claims.
A composition substantially as described herein with reference to the description and figures.
A medical implant or device, or biomaterial for use in the same, substantially as described herein with reference to the description and figures.
Use of a composition substantially as described herein with reference to the description and figures.
A method for treating or preventing infection substantially as described herein with reference to the description and figures.
A method for treating wounds substantially as described herein with reference to the description and figures.
Dependencies are inferred from the claim text.