Analysis method and analysis device
AssigneeHitachi High-Tech Corporation
InventorHiroaki NAKAGAWA
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Abstract
Provided is a high-accuracy analysis method utilizing an enzyme-lined immunoassay. The presence of an analyte 3 can be detected or the abundance of the analyte 3 can be analyzed by: bonding an antibody 5 that is capable of specifically bonding to the analyte 3 immobilized on a solid phase 1 and has an enzyme 7 bonded thereto; then decomposing an enzyme substrate 8, which can generate decomposition products capable of being detected easily with a mass spectrometry, with the enzyme 7 bonded to the antibody 5; and then analyzing the decomposition products 9 and 10 with a mass spectrometry.
Claims5
An analysis method for measuring an analyte,
- wherein an analyte is immobilized on a solid phase,
- wherein an antibody to which a label to be specifically bonded to the analyte immobilized on the solid phase is bonded to the analyte,
- wherein a label bonded substance having galactosidase bonded thereto is bonded to the label,
- wherein enzyme reaction is caused to occur between the galactosidase bonded to the label bonded substance and an enzyme substrate, and
- wherein mass spectrometry is performed on an enzyme reaction product of the obtained enzyme substrate so as to measure the presence or absence and concentration of the analyte.
Dependencies are inferred from the claim text.