Aminoacyl-trna synthetase efficiently introducing lysine derivatives
AssigneeSuzhou Kunpeng Biotech Co., Ltd.
InventorsHuiling LIU, Song WU, Zhenshan ZHANG, Wei CHEN
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Abstract
Provided by the present invention is a mutant lysyl-tRNA synthetase that has a deletion mutation at one or more sites selected from the group corresponding to the amino acid sequence of wild-type aminoacyl-tRNA synthetase: 102nd, 128th-140th, and 159th-179th amino acid residues. Compared with wild-type lysyl-tRNA synthetase, the mutant lysyl-tRNA synthetase according to the present invention has high activity, a high level of expression and good solubility, and may significantly increase the amount of an inserted unnatural amino acid and the level of expression of a target protein containing an unnatural amino acid.
Claims17
A mutant protein of aminoacyl-tRNA synthetase, which comprises a deletion mutation of amino acid residue at one or more positions selected from the group consisting of:
- position 102, positions 128-140, and positions 159-179 in an amino acid sequence corresponding to a wild-type aminoacyl-tRNA synthetase.
9-10. (canceled)
An expression system, which comprises:
- (a) the mutant protein according to the first aspect of the present invention;
- and
- (b) an artificial tRNA capable of binding to a predetermined modified amino acid in the presence of the mutant protein;
- and optionally
- (c) a first nucleic acid sequence encoding a target protein, wherein the first nucleic acid sequence comprises a codon recognized by the artificial tRNA.
Dependencies are inferred from the claim text.