Conversion of lignin-derived monomers to muconate by engineered pseudomonas
AssigneeAlliance for Sustainable Energy, LLC
InventorsChristopher W. JOHNSON, Gregg Tyler BECKHAM, Allison Jean ZIMONT WERNER
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Abstract
Disclosed herein are engineered Pseudomonas useful to relieve the metabolic bottleneck of 4-hydroxybenzoate transformation in a muconate accumulating strain on an engineered Pseudomonas by swapping its endogenous para-hydroxybenzoate-3-hydroxylase (PHBH), PobA, with a homolog, PraI, that has a broader cofactor preference.
Claims18
An engineered Pseudomonas useful to relieve the metabolic bottleneck of 4-hydroxybenzoate transformation in an engineered Pseudomonas by swapping its endogenous para-hydroxybenzoate-3-hydroxylase (PHBH), PobA, with PraI.
An engineered Pseudomonas useful to relieve the metabolic bottleneck of 4-hydroxybenzoate transformation in an engineered Pseudomonas comprising PraI.
An engineered Pseudomonas capable of production of beta-ketoadipic acid useful to relieve the metabolic bottleneck of 4-hydroxybenzoate transformation in the engineered Pseudomonas comprising PraI.
Dependencies are inferred from the claim text.