Efficient method for preparing ppr protein and use of the same

A PPR motif, which is any one of the following PPR motifs:

• (A-1) a PPR motif consisting of the sequence of SEQ ID NO:
• 9, or a PPR motif consisting of the sequence of SEQ ID NO:
• 9 having a substitution selected from the group consisting of substitution of the amino acid at position 10 with tyrosine, substitution of the amino acid at position 15 with lysine, substitution of the amino acid at position 16 with leucine, substitution of the amino acid at position 17 with glutamic acid, substitution of the amino acid at position 18 with aspartic acid, and substitution of the amino acid at position 28 with glutamic acid, or a PPR motif consisting of the sequence of SEQ ID NO:
• 401 or a PPR motif consisting of the sequence of SEQ ID NO:
• 401 having a substitution selected from the group consisting of substitution of the amino acid at position 10 with tyrosine, substitution of the amino acid at position 16 with leucine, substitution of the amino acid at position 17 with glutamic acid, substitution of the amino acid at position 18 with aspartic acid, and substitution of the amino acid at position 28 with glutamic acid;
• (A-2) a PPR motif consisting of the sequence of SEQ ID NO:
• 9 or 401 having a substitution, deletion, or addition of 1 to 20 amino acids other than the amino acids at positions 1, 2, 3, 4, 6, 7, 9, 11, 12, 14, 19, 26, 30, 33, and 34, and having an adenine-binding property;
• (A-3) a PPR motif having a sequence identity of at least 42% to the sequence of SEQ ID NO:
• 9 or 401, provided that the amino acids at positions 1, 2, 3, 4, 6, 7, 9, 11, 12, 14, 19, 26, 30, 33, and 34 are identical, and having an adenine-binding property;
• (C-1) a PPR motif consisting of the sequence of SEQ ID NO:
• 10, or a PPR motif consisting of the sequence of SEQ II) NO:
• 10 having a substitution of amino acid selected from the group consisting of substitution of the amino acid at position 2 with serine, substitution of the amino acid at position 5 with isoleucine, substitution of the amino acid at position 7 with leucine, substitution of the amino acid at position 8 with lysine, substitution of the amino acid at position 10 with phenylalanine or tyrosine, substitution of the amino acid at position 15 with arginine, substitution of the amino acid at position 22 with valine, substitution of the amino acid at position 24 with arginine, substitution of the amino acid at position 27 with leucine, and substitution of the amino acid at position 29 with arginine;
• (C-2) a PPR motif consisting of the sequence of SEQ ID NO:
• 10 having a substitution, deletion, or addition of 1 to 2.5 amino acids other than the amino acids at positions 1, 3, 4, 14, 18, 19, 26, 30, 33, and 34, and having a cytosine-binding property;
• (C-3) a PPR motif having a sequence identity of at least 25% to the sequence of SEQ. ID NO:
• 10, provided that the amino acids at positions 1, 3, 4, 14, 18, 19, 26, 30, 33, and 34 are identical, and having a cytosine-binding property;
• (G-1) a PPR motif consisting of the sequence of SEQ ID NO:
• 11, or a PPR motif consisting of the sequence of SEQ ID NO:
• 11 having a substitution selected from the group consisting of substitution of the amino acid at position 10 with phenylalanine, substitution of the amino acid at position 15 with aspartic acid, substitution of the amino acid at position 27 with value, substitution of the amino acid at position 28 with serine, and substitution of the amino acid at position 35 with iso leucine;
• (G-2) a PPR motif consisting of the sequence of SEQ ID NO:
• 11 having a substitution, deletion, or addition of 1 to 21 amino acids other than the amino acids at positions 1, 2, 3, 4, 6, 7, 9, 14, 18, 19, 26, 30, 33, and 34, and having a guanine-binding property;
• (G-3) a PPR motif having a sequence identity of at least 40% to the sequence of SEQ ID NO:
• 11, provided that the amino acids at positions 1, 2, 3, 4, 6, 7, 9, 14, 18, 19, 26, 30, 33, and 34 are identical, and having a guanine-binding property;
• (U-1) a PPR motif consisting of the sequence of SEQ ID NO:
• 12, or a PPR motif consisting of the sequence of SEQ ID NO:
• 12 having a substitution selected from the group consisting of substitution of the amino acid at position 10 with phenylalanine, substitution of the amino acid at position 13 with serine, substitution of the amino acid at position 15 with lysine, substitution of the amino acid at position 17 with glutamic acid, substitution of the amino acid at position 20 with leucine, substitution of the amino acid at position 21 with lysine, substitution of the amino acid at position 23 with phenylalanine, substitution of the amino acid at position 24 with aspartic acid, substitution of the amino acid at position 27 with lysine, substitution of the amino acid at position 2.8 with lysine, substitution of the amino acid at position 2.9 with arginine, and substitution of the amino acid at position 31 with leucine;
• (U-2) a PPR motif consisting of the sequence of SEQ ID NO:
• 12 having a substitution, deletion, or addition of 1 to 22 amino acids other than the amino acids at positions 1, 2, 3, 4, 6, 11, 12, 14, 19, 26, 30, 33, and 34, and having a uracil-binding property;
• and
• (U-3) a PPR motif having a sequence identity of at least 37% to the sequence of SEQ ID NO:
• 12, provided that the amino acids at positions 1, 2, 3, 4, 6, 11, 12, 14, 19, 26, 30, 33, and 34 are identical, and having a uracil-binding property.