Methods and devices for detection of coagulation impairment
OwnerGalit H. Frydmaninventor
Life of this patent
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Abstract
Provided are methods and devices for evaluating coagulation, including the identification of a coagulation impairment such as a factor deficiency or the presence of a factor inhibitor. In various embodiments, the methods and devices measure coagulation of a sample in response to the addition of one or more coagulation factors, added at various concentrations to portions of the sample. Such coagulation measurements can be evaluated to accurately profile coagulation impairments of the sample. In additional various embodiments, point-of-care or bedside testing with a convenient, microfluidic device can be used by minimally trained personnel.
Claims28
1-47. (canceled)
A method of assessing coagulation in a sample from a subject, the method comprising:
- adding Factor IIa to a first portion of the sample;
- adding Factor I and Factor IIa to a second portion of the sample;
- and
- measuring clot formation for the first and second portions of the sample.
A method of assessing coagulation in a sample from a subject, the method comprising:
- adding Factor I to two or more first portions of the sample, each first portion receiving the Factor I at a different concentration;
- adding Factor IIa to two or more second portions of the sample, each second portion receiving the Factor IIa at a different concentration;
- adding Factor I and Factor IIa to two or more third portions of the sample, each third portion receiving the Factor I at a different concentration and receiving the Factor IIa at the same concentration;
- and
- measuring clot formation for each of the first, second, and third portions of the sample.
A method of assessing coagulation in a blood sample, the method comprising:
- adding antithrombin III and Factor Xa, or antithrombin III and Factor IIa, to a first portion of the blood sample;
- adding Activated Protein C to a second portion of the blood sample;
- and
- measuring clot formation for the first and second portions of the blood sample.
A method of assessing whether a patient suffers from a Factor V impairment, the method comprising:
- adding antithrombin III to two or more first portions of a blood sample from the patient, each first portion receiving the antithrombin III at a different concentration;
- adding Activated Protein C to two or more second portions of the blood sample, each second portion receiving the Activated Protein C at a different concentration;
- adding Activated Protein C and Factor V to at least one third portion of the blood sample;
- and
- measuring clot formation for each of the first, second, and third portions of the blood sample.
A method of assessing whether a patient suffers from a Factor V impairment, the method comprising:
- adding antithrombin III to two or more first portions of a blood sample from the patient, each first portion receiving the antithrombin III at a different concentration;
- adding Activated Protein C to two or more second portions of the blood sample, each second portion receiving the Activated Protein C at a different concentration;
- adding Protein S and Factor V to at least one third portion of the blood sample;
- and
- measuring clot formation for each of the first, second, and third portions of the blood sample.
A microfluidic device for detecting coagulation in a sample from a subject, the microfluidic device comprising:
- a first group of channels comprising Factor I at increasing concentrations across channels of the first group;
- a second group of channels comprising Factor IIa at increasing concentrations across channels of the second group;
- and
- a third group of channels comprising Factor I at increasing concentrations across channels, and comprising Factor IIa at the same concentration across channels of the third group,
- wherein each channel in each of the first, second, and third groups of channels comprises a clot forming area that localizes clot formation, and
- wherein each of the first, second, and third groups of channels comprises at least two channels.
Dependencies are inferred from the claim text.