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US 2022/0282302 A1Published application
Linearly-amplified internal control for nucleic acid amplification reaction
AssigneeGenomadix Inc.
InventorsChris Harder, Jeffrey Do, Andy Lee
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Abstract
The present disclosure provides, among other things, systems, methods, and kits include internal amplification controls. Provided internal amplification controls are or include non-target sequences that are amplified during nucleic acid amplification. Provided internal amplification controls are linearly amplified. Provided internal amplification controls are useful for systems, methods and kits for nucleic acid amplification.
Claims12
-13. (canceled)
An internal amplification control for a nucleic acid amplification reaction, comprising:
- a single oligonucleotide primer;
- and
- a nucleic acid template,
- wherein when present with a target nucleic acid sample and contacted with a nucleic acid amplification reagent in a reaction vessel, the nucleic acid template linearly amplifies and the target nucleic acid sample exponentially or linearly amplifies, and
- wherein when the nucleic acid template is present at a higher concentration than the target nucleic acid sample, amplification of the nucleic acid template does not consume nucleic acid amplification reagents at a faster rate than amplification of the target nucleic acid sample.
2 more elements
Dependencies are inferred from the claim text.