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US 2022/0282291 A1Published application
Cell-free production of sugars
AssigneeGreenLight Biosciences, Inc.
InventorsDaniel MacEachran, Drew S. Cunningham, William Jeremy Blake, Matthew Eduardo Moura
Life of this patent
Estimating the term…
Abstract
Provided herein, in some embodiments, are systems, methods, and compositions (e.g., cells and cell lysates) for enzymatically converting a polymeric glucose carbohydrate (e.g., starch) to sugar.
Claims21
1-68. (canceled)
A method for producing allulose, the method comprising:
- converting fructose 6-phosphate (F6P) to allulose-6-phosphate (A6P), catalyzed by an allulose 6-phosphate epimerase (A6PE) selected from the group consisting of:
- Brevibacillus thermoruber A6PE, Thermoanaerobacter brockii A6PE, Caldanaerobacter subterraneus A6PE, Deferribacter desulfuricans A6PE, Thermocrinis ruber A6PE, Hydrogenivirga sp. 128-5-R1-1 A6PE, Thermosipho atlanticus A6PE, and Thermosulfidibacter takaii A6PE;
- and
- converting A6P to allulose, catalyzed by an allulose 6-phosphate phosphatase (A6PP).
1 more element
A method for producing allulose, the method comprising:
- converting fructose 6-phosphate (F6P) to allulose 6-phosphate (A6P), catalyzed by the A6PE, and
- converting A6P to allulose, catalyzed by the A6PP, in a cell lysate mixture comprising cell lysates from microbial cells that express one or both of an allulose 6-phosphate epimerase (A6PE) and an allulose 6-phosphate phosphatase (A6PP),
- wherein the A6PE is derived from an organism selected from the group consisting of:
- Brevibacillus thermoruber, Thermoanaerobacter brockii, Caldanaerobacter subterraneus, Deferribacter desulfuricans, Thermocrinis ruber, Hydrogenivirga sp. 128-5-R1-1, Thermosipho atlanticus, and Thermosulfidibacter takaii.
1 more element
A method for producing allulose, the method comprising:
- converting fructose 6-phosphate (F6P) to allulose-6-phosphate (A6P), catalyzed by an allulose 6-phosphate epimerase (A6PE);
- and
- converting A6P to allulose, catalyzed by an allulose 6-phosphate phosphatase (A6PP) selected from the group consisting of:
- Ruminiclostridium thermocellum A6PP, Acidothermus cellulolyticus A6PP, Aquifex aeolicus A6PP, Bacteroides vulgatus A6PP, Caldanaerobacter subterraneus A6PP, Crenarchaeota archaeon 13_I_40CM_3_53_5 A6PP, Deinococcus geothermalis DSM 11300 A6PP, Desulfotomaculum kuznetsovii A6PP, Methanothermobacter thermautotrophicus A6PP, Petrotoga mobilis A6PP, Pyrococcus horikoshii Ot3 A6PP, Thermoanaerobacter ethanolicus A6PP, Thermoanaerobacter wiegelii A6PP, Thermobifida fusca A6PP, Thermodesulfatator indicus A6PP, Thermosphaera aggregans A6PP, Thermotoga neapolitana A6PP, Thermus islandicus A6PP, Thermus thermophilus A6PP.
1 more element
Dependencies are inferred from the claim text.