Recombinant microorganisms and uses therefor
AssigneeLanzaTech, Inc.
InventorsShivani Garg, Michael Koepke
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Abstract
Microorganisms are genetically engineered to produce various chemicals for industrial use. The microorganisms are carboxydotrophic acetogens. The microorganisms produce acetyl-CoA using the Wood-Ljungdahl Pathway for fixing CO/CO2. A reverse beta-oxidation pathway cycle from a microorganism that contains such a group of enzymes is introduced. Additionally, primers and extenders, and/or genes encoding for enzymes that generate primers and extenders may also be introduced. Product synthesis can be effected by improved promoters or enzyme designs that are catalytically more efficient. Similarly, product synthesis may also be improved by deleting competing reactions.
Claims20
A genetically engineered microorganism capable of producing a product from a gaseous substrate, wherein the microorganism comprises an iterative pathway comprising:
- a) a nucleic acid encoding a group of enzymes that are capable of catalyzing the conversion of (Cn)-acyl CoA to β-ketoacyl-CoA;
- b) a nucleic acid encoding a group of exogenous enzymes capable of catalyzing the conversion of β-ketoacyl-CoA to β-hydroxyacyl-CoA;
- c) a nucleic acid encoding a group of exogenous enzymes capable of catalyzing the conversion of β-hydroxyacyl-CoA to trans-Δ2-Enoyl-CoA;
- d) a nucleic acid encoding a group of exogenous enzymes capable of catalyzing the conversion of trans-Δ2-Enoyl-CoA to (Cn+2) acyl-CoA;
- e) one or more termination enzymes;
- and
- wherein the microorganism is a C1-fixing bacteria comprising a disruptive mutation in a thioesterase.
Dependencies are inferred from the claim text.