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US 2022/0280887 A1Published application
Methods for purifying antibodies
AssigneeUcb Biopharma Srl
InventorsNEIL ALAN WATSON, CURTIS WILLIAM PHIPPEN
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Abstract
The invention relates to the field of manufacturing recombinant antibody molecules. In particular, methods of purifying such recombinant antibody molecules are provided wherein imidazole or an imidazole-analogue is added during the elution of the recombinant antibody molecule from an affinity chromatography resin, such as a protein-A-based resin.
Claims10
1-9. (canceled)
A method for purifying an antibody comprising:
- a) loading a mixture comprising the antibody to be purified onto an affinity chromatography resin,
- wherein the affinity chromatography resin is not a nickel-based, zinc-based or cobalt-based resin;
- b) washing the chromatographic resin with a wash buffer;
- c) eluting the antibody with an elution buffer that comprises 0.01M to 1.0M imidazole or an imidazole-analogue and a pH of 3 to 5.
1 more element
Dependencies are inferred from the claim text.