Methods for making recombinant protein
AssigneeMonash University
InventorsJames Whisstock, Ruby Law, Adam Quek, Paul Conroy
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Abstract
The present invention relates to methods of producing recombinant plasminogen in a mammalian expression system. A method for producing plasminogen, the method comprising (i) providing a host cell comprising a first recombinant polynucleotide encoding plasminogen and a second recombinant polynucleotide encoding a plasminogen activation inhibitor; (ii) culturing said host cell in a suitable culture medium under conditions to effect expression of plasminogen from the first polynucleotide and plasminogen activation inhibitor from the second polynucleotide.
Claims34
A method for producing plasminogen, the method comprising:
- (i) providing a host cell comprising a first recombinant polynucleotide encoding plasminogen and a second recombinant polynucleotide encoding plasminogen activator inhibitor-1 (PAI-1);
- (ii) culturing said host cell in a suitable culture medium under conditions to effect expression of plasminogen from the first polynucleotide and PAI-1 from the second polynucleotide.
A method of producing recombinant plasminogen, the method comprising the steps of:
- (a) providing a first polynucleotide encoding plasminogen,
- (b) providing a second polynucleotide encoding PAI-1,
- wherein the first and the second polynucleotide are operably linked to a promoter for enabling the expression of the polynucleotides,
- (c) providing a host cell,
- (d) transforming or transfecting the host cell with the polynucleotides of (a) and (b),
- (e) providing cell culture media,
- (f) culturing the transformed or transfected host cell in the cell culture media under conditions sufficient for expression of plasminogen and PAI-1, and
- optionally (g) recovering or purifying plasminogen from the host cell and/or the cell culture media.
A method for producing plasminogen, the method comprising:
- (i) providing a host cell comprising a recombinant polynucleotide encoding plasminogen;
- (ii) culturing said host cell in a suitable culture medium under conditions to effect expression of plasminogen from the polynucleotide,
- wherein the culture media comprises PAI-1.
A method of producing recombinant plasminogen, the method comprising the steps of:
- (a) providing a polynucleotide encoding plasminogen,
- wherein the polynucleotide is operably linked to a promoter for enabling the expression of the polynucleotide,
- (c) providing a host cell,
- (d) transforming or transfecting the host cell with the polynucleotide of (a)
- (e) providing cell culture media comprising PAI-1,
- (f) culturing the transformed or transfected host cell in the cell culture media under conditions sufficient for expression of plasminogen, and
- optionally (g) recovering or purifying plasminogen from the host cell and/or the cell culture media.
A vector or nucleic acid construct comprising a first polynucleotide sequence encoding plasminogen and a second polynucleotide sequence encoding PAI-1.
Dependencies are inferred from the claim text.